Probiotics and Pet Health

What are probiotics?

Probiotics are formulas that contain living microorganisms, usually bacteria (but sometimes other microorganisms such as yeast), that are consumed to improve health. The goal of ingesting probiotic substances is to bring the gut microbiota back to a state of balance after it has been disrupted by antibiotic treatment or other disturbances. However, probiotics can also be used to maintain a healthy gut and prevent gastrointestinal issues¹. Most probiotic organisms are lactic-acid bacteria^2,3, which means they can ferment (or break down) sugars into lactic acid. It is thought that these species colonize the intestine during early life^4,5 and allow other symbiotic bacteria to take residence as an individual grows into adulthood.

Scientists are currently exploring the utility of probiotics to treat conditions such as diarrhea⁶, inflammatory bowel disease⁷, and lactose-intolerance⁸ (in humans). The underlying idea is to introduce bacterial strains that can compete with those that are overgrown and contributing to health issues. Bacteria can contend with each other in a variety of ways⁹, including competition for nutrients or the production of substances that thwart the growth of rival species. The desired result is the resolution of symptoms and a balanced microbiota, where disruptive strains are reduced to normal levels. Humans can find probiotics in foods like yogurts and cheese in addition to dietary supplements. Probiotics for dogs and cats are typically sold in the form of pills or powders that can be added to their food.

What bacteria are in probiotics?

The most common bacteria used in dog and cat probiotics¹⁰ are Lactobacillus, a genus of lactic-acid bacteria^2,3 that includes regular inhabitants of the gastrointestinal tract^11,12. Some Lactobacilli strains are thought to improve health by preventing the colonization of pathogenic bacteria, including Clostridium species¹⁰. They are also able to survive acidic conditions, such as those encountered in the stomach, which is necessary for them to remain viable as they transit from the mouth to the gastrointestinal tract. Other strains often found in probiotic formulas belong to the genera Enterococcus and Bifidobacterium^10,12. Some species of the latter have been associated with the early colonization of the infant gut microbiome in mice and humans¹³.

Examples of bacterial species used in companion animal probiotic formulas are Enterococcus faecium, Lactobacillus acidophilus, and Bifidobacterium animalis^5,10,14. Lactobacilli have also been used for centuries in the production of fermented foods (think cheese and yogurt) and are important for digestion of lactose and some essential vitamins^2,3. Since probiotic use in pets is still a growing area of study, the exact role each individual strain plays in improving intestinal health is not fully understood. However, these species are often isolated from healthy animals¹⁴.

How can probiotics be enhanced?

Research has shown that many probiotic strains of bacteria are able to establish themselves in the gastrointestinal tract, alter the compositions of gut microbes and metabolites, and modulate host immunity^15,16—all key traits for colonization of the gut and changing the composition of the microbiome. Nonetheless, some studies have found positive effects in the absence of these traits. Two other components can be used in conjunction with probiotics in an effort to increase these traits, called prebiotics and synbiotics.

Prebiotics¹⁴ are a combination of molecules that help promote the growth of beneficial gut bacteria that are included in probiotics. A couple common examples are types of fiber, such as inulin, and sugar molecules called fructo-oligosaccharides (FOS). These nutrients are digested by bacteria like Lactobacilli or Enterococcus and act to give these species a competitive advantage over others currently inhabiting the gut.

Synbiotics¹⁴ are a combination of prebiotics and probiotics. They contain both live bacteria and the necessary components to promote their growth in the body. The objective is that as bacteria are introduced into the intestinal tract, the required nutrients are presented simultaneously to increase their odds of successful colonization. A previous study demonstrated that a synbiotic decreased time to resolution of diarrhea symptoms in dogs compared to a placebo, indicating that synbiotics may help in recovery. However, this was not tested against the probiotic alone and requires further inquiry¹⁷.  

How do probiotics influence health?

Probiotics have been widely tested for their effects on health. While it is clear is that some do have health benefits, we still do not completely understand how this is achieved. Furthermore, there are differences between studies¹⁰ regarding their direct impact on well-being. Nevertheless, probiotics have been shown to successfully treat acute gastroenteritis, irritable bowel syndrome, and to prevent allergy¹⁰. Their use may also reduce the recovery time following acute diarrhea¹⁷.

How might probiotics support health? One possibility is by outcompeting pathogenic microbes for food or colonization sites. It might also be through stimulating their host’s immune system to decrease inflammation in the gut or help recognize invaders. Probiotics could further change the metabolism of other bacteria or produce antimicrobial substances that kill their competitors. Future research will help outline the mechanisms and better dictate how probiotics should be used to improve intestinal health¹⁸.

The Nom Nom R&D team is on the lookout for dogs that experience regular diarrhea to participate in their new GI microbiome study. Sound like someone in your house? See if your dog qualifies for this paid research study here.

References

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2. Todar, K., Madison & WI. Lactic Acid Bacteria. Available at: http://textbookofbacteriology.net/lactics.html. (Accessed: 5th December 2018)
3. Biotechnology of lactic acid bacteria: novel applications. (2016).
4. Moles, L. et al. Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life. PLoS One 8, e66986 (2013).
5. Barko, P. C., McMichael, M. A. & Swanson, K. S. The gastrointestinal microbiome: a review. Journal of veterinary (2018).
6. Gómez-Gallego, C. et al. A canine-specific probiotic product in treating acute or intermittent diarrhea in dogs: A double-blind placebo-controlled efficacy study. Vet. Microbiol. 197, 122–128 (2016).
7. White, R. et al. Randomized, controlled trial evaluating the effect of multi-strain probiotic on the mucosal microbiota in canine idiopathic inflammatory bowel disease. Gut Microbes 8, 451–466 (2017).
8. Oak, S. J. & Jha, R. The effects of probiotics in lactose intolerance: A systematic review. Crit. Rev. Food Sci. Nutr. 1–9 (2018). doi:10.1080/10408398.2018.1425977
9. Hibbing, M. E., Fuqua, C., Parsek, M. R. & Peterson, S. B. Bacterial competition: surviving and thriving in the microbial jungle. Nat. Rev. Microbiol. 8, 15–25 (2010).
10. Grześkowiak, Ł., Endo, A., Beasley, S. & Salminen, S. Microbiota and probiotics in canine and feline welfare. Anaerobe 34, 14–23 (2015).
11. Deng, P. & Swanson, K. S. Gut microbiota of humans, dogs and cats: current knowledge and future opportunities and challenges. Br. J. Nutr. 113 Suppl, S6–17 (2015).
12. Azad, M. A. K., Sarker, M., Li, T. & Yin, J. Probiotic Species in the Modulation of Gut Microbiota: An Overview. Biomed Res. Int. 2018, 9478630 (2018).
13. O’Callaghan, A. & van Sinderen, D. Bifidobacteria and Their Role as Members of the Human Gut Microbiota. Front. Microbiol. 7, 925 (2016).
14. Schmitz, S. & Suchodolski, J. Understanding the canine intestinal microbiota and its modification by pro-, pre- and synbiotics - what is the evidence? Vet Med Sci 2, 71–94 (2016).
15. Strompfová, V., Kubašová, I. & Lauková, A. Health benefits observed after probiotic Lactobacillus fermentum CCM 7421 application in dogs. Appl. Microbiol. Biotechnol. 101, 6309–6319 (2017).
16. Garcia-Mazcorro, J. F. et al. Effect of a multi-species synbiotic formulation on fecal bacterial microbiota of healthy cats and dogs as evaluated by pyrosequencing. FEMS Microbiol. Ecol. 78, 542–554 (2011).
17. Gagné, J. W. et al. Effects of a synbiotic on fecal quality, short-chain fatty acid concentrations, and the microbiome of healthy sled dogs. BMC Vet. Res. 9, 246 (2013).
18. Walker, W. A. Mechanisms of action of probiotics. Clin. Infect. Dis. 46 Suppl 2, S87–91; discussion S144–51 (2008).